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二氫硫辛醯胺脫氫酶

維基百科，自由的百科全書
二氫硫辛醯胺脫氫酶
PDB渲染的1zy8
有效結構
PDB 直系同源檢索：PDBe, RCSB
標識
代號 DLD; DLDH; E3; GCSL; LAD; PHE3
擴展標識 遺傳學：238331 鼠基因：107450 同源基因：84 GeneCards: DLD Gene
EC編號 1.8.1.4
直系同源體
物種 人類 小鼠
Entrez 1738 13382
Ensembl ENSG00000091140 ENSMUSG00000020664
UniProt P09622 O08749
mRNA序列 NM_000108 NM_007861.4
蛋白序列 NP_000099 NP_031887.2
基因位置 Chr 7:
107.53 – 107.57 Mb		 Chr 12:
32.02 – 32.04 Mb
PubMed查詢 [1] [2]
二氫硫辛醯胺脫氫酶
識別碼
EC編號 1.8.1.4
CAS號 9001-18-7
資料庫
IntEnz IntEnz瀏覽
BRENDA（英語：BRENDA） BRENDA入口
ExPASy（英語：ExPASy） NiceZyme瀏覽
KEGG KEGG入口
MetaCyc（英語：MetaCyc） 代謝路徑
PRIAM（英語：PRIAM_enzyme-specific_profiles） 概述
PDB RCSB PDB PDBj PDBe PDBsum
基因本體 AmiGO / EGO

二氫硫辛醯胺脫氫酶（EC 1.8.1.4，英語：Dihydrolipoamide dehydrogenase，縮寫DLD，又稱為粒線體二氫硫辛酸脫氫酶，dihydrolipoyl dehydrogenase, mitochondrial）是一種由人類基因DLD[1][2][3][4]所編碼的黃素蛋白，其作用是將二氫硫辛醯胺脫氫從而轉化為氧化型硫辛醯胺。

DLD作為一種粒線體蛋白質，在真核生物的能量代謝中起到重要作用，它至少參與了五種多酶複合體，且為複合體完成反應所必需的組份[5]。另外，DLD作為一種黃素蛋白氧化還原酶，以FAD為輔基接受質子與電子催化二硫鍵的形成。

DLD是大小為51千道爾頓亞基的同二聚體，其中每個亞基都與一分子的FAD以共價鍵的形式相連[6]。

功能

[編輯]

在哺乳動物的粒線體三羧酸循環中，該酶與丙酮酸脫氫酶和二氫硫辛醯基乙醯基轉基酶一起組成中丙酮酸脫氫酶複合體，同時該酶亦是α-酮戊二酸脫氫酶複合體中的一個組分。二氫硫辛醯胺脫氫酶的主要任務是負責將複合物中另一組分硫辛醯胺轉乙醯基酶（或二氫硫辛醯琥珀醯轉移酶）的輔基二氫硫辛醯胺脫氫從而轉化為氧化型硫辛醯胺，該酶也是粒線體甘氨酸剪切體系的組分。

參考文獻

[編輯]
  1. ^ Entrez Gene: dihydrolipoamide dehydrogenase. （原始內容存檔於2010-03-06）. 
  2. ^ Otulakowski G, Robinson BH. Isolation and sequence determination of cDNA clones for porcine and human lipoamide dehydrogenase. Homology to other disulfide oxidoreductases. J. Biol. Chem. December 1987, 262 (36): 17313–8. PMID 3693355. 
  3. ^ Pons G, Raefsky-Estrin C, Carothers DJ, Pepin RA, Javed AA, Jesse BW, Ganapathi MK, Samols D, Patel MS. Cloning and cDNA sequence of the dihydrolipoamide dehydrogenase component human alpha-ketoacid dehydrogenase complexes. Proc. Natl. Acad. Sci. U.S.A. March 1988, 85 (5): 1422–6. PMC 279783 可免費查閱. PMID 3278312. doi:10.1073/pnas.85.5.1422. 
  4. ^ Scherer SW, Otulakowski G, Robinson BH, Tsui LC. Localization of the human dihydrolipoamide dehydrogenase gene (DLD) to 7q31----q32. Cytogenet. Cell Genet. 1991, 56 (3-4): 176–7. PMID 2055113. doi:10.1159/000133081. 
  5. ^ Babady NE, Pang YP, Elpeleg O, Isaya G. Cryptic proteolytic activity of dihydrolipoamide dehydrogenase. Proceedings of the National Academy of Sciences of the United States of America. 2007, 104 (15): 6158–63. PMC 1851069 可免費查閱. PMID 17404228. doi:10.1073/pnas.0610618104. 
  6. ^ Ciszak EM, Makal A, Hong YS, Vettaikkorumakankauv AK, Korotchkina LG, Patel MS. How dihydrolipoamide dehydrogenase-binding protein binds dihydrolipoamide dehydrogenase in the human pyruvate dehydrogenase complex. The Journal of Biological Chemistry. 2006, 281 (1): 648–55. PMID 16263718. doi:10.1074/jbc.M507850200. 

深入閱讀

[編輯]
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外部連結

[編輯]

二氫硫辛醯胺脫氫酶引用了美國國家醫學圖書館提供的資料，這些資料屬於公共領域。


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