BeWo
BeWo (亦称ATCC CCL-98) 是人类胎盘绒毛膜癌细胞系[1]，较常应用于胎盘屏障生理功能[2]及人类免疫缺陷病毒的研究[3]，而超三倍体JEG-3细胞则是其衍生的克隆。BeWo细胞具有可产生大量层粘连蛋白的细胞外基质[4]，并且BeWo细胞会表达雌激素 (雌酮、雌二醇及雌三醇)、孕激素、人绒毛膜促性腺激素、胎盘催乳素及角蛋白等物质。目前已知BeWo的b30亚克隆可在双房室 (bicameral chambers) 的可渗透膜上生长，以形成融合性的细胞层，从而确定营养从顶端表面吸收的速率，以及从基底外侧膜中流出的速率[5]。
有研究指出沉默LOX在Bewo细胞中的表达，会明显降低Bewo细胞侵袭及转移能力。因为过度表达LOX可以改变肿瘤细胞外基质的结构，并且激活特定通路及上调MMPs类的表达，使乳癌的转移侵袭速度因基底膜被降解而加速[6]。
参考资料[编辑]
- ^ Pattillo, RA; Gey, GO. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro.. Cancer research. 1968-07, 28 (7): 1231–6 [2019-12-08]. PMID 4299001.
- ^ Zhou, F; Hong, M; You, G. Regulation of human organic anion transporter 4 by progesterone and protein kinase C in human placental BeWo cells.. American journal of physiology. Endocrinology and metabolism. 2007-07, 293 (1): E57–61 [2019-12-08]. PMID 17341544. doi:10.1152/ajpendo.00696.2006.
- ^ Ayouba, A; Cannou, C; Nugeyre, MT; Barré-Sinoussi, F; Menu, E. Distinct efficacy of HIV-1 entry inhibitors to prevent cell-to-cell transfer of R5 and X4 viruses across a human placental trophoblast barrier in a reconstitution model in vitro.. Retrovirology. 2008-03-31, 5: 31 [2019-12-08]. PMID 18377645. doi:10.1186/1742-4690-5-31.
- ^ Church, HJ; Aplin, JD. BeWo choriocarcinoma cells produce laminin 10.. The Biochemical journal. 1998-06-01,. 332 ( Pt 2): 491–8 [2019-12-08]. PMID 9601079. doi:10.1042/bj3320491.
- ^ Heaton, SJ; Eady, JJ; Parker, ML; Gotts, KL; Dainty, JR; Fairweather-Tait, SJ; McArdle, HJ; Srai, KS; Elliott, RM. The use of BeWo cells as an in vitro model for placental iron transport.. American journal of physiology. Cell physiology. 2008-11, 295 (5): C1445–53 [2019-12-08]. PMID 18815225. doi:10.1152/ajpcell.00286.2008.
- ^ 王璟; 郭影; 赵向寨; 李萍. LOX基因對人絨癌Bewo細胞侵襲轉移的影響及意義. 中国老年学杂志. 2014, (11): 3074–6. doi:10.3969/j.issn.1005-9202.2014.11.076.